|
KMID : 0357319950300030367
|
|
Journal of the Korean Society for Microbiology
1995 Volume.30 No. 3 p.367 ~ p.374
|
|
|
Mechanism of Induction of Liver Tumors by Non-genotoxic Chemicals
|
|
신부안/Ramareddy V. Guntaka
|
|
|
Abstract
|
|
|
Dichloroacetic acid(DCA) is a principal by-product of the chlorine disinfection of water containing humic and fulvic acids, and is also a drug of interest in the therapeutic management of metabolic disorders. DCA has recently been shown to
increase
significantly the incidence of hepatic adenomas and hepatocellular carcinomas in male B6C3F1 mice. Although little is known about the mechanism of DCA carcinogenesis, chronic ingestion of the compound in drinking water induces primarily
hyperplastikc
nodules prior to the appearance of hepatic adenomas and hepatocellular carcinomas. The present study was undertaken to isolate and identify the specific genes from DCA-treated mouse liver by using subtraction hybridization method. We isolated
cDNA
(cdca2m) expressed much higher in DCA-treated hepatic tissues than normal. Cdca2m shows 98.7% homology with rat S-adenosylmethionine (AdoMet) synthetase mRNA and 82.4% homology with human mRNA for AdoMet synthetase.
To our suprise, we also found the expression of cdca2m was dramatically reduced in DCA-induced hepatic carcinoma. These results suggest that AdoMet synthetase may contribute some important roles in carcinogenesis of DCA.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|